血管内皮生长因子在次声作用后的表达及其与血-视网膜屏障破坏的关系

Effect of infrasound on the expression of VEGF in the retina and its relation to breakdown of blood-retinal barrier

摘要:

目的探讨血管内皮生长因子(VEGF)在次声暴露后大鼠视网膜的表达及其与次声暴露的关系.方法免疫组织化学法研究不同时间次声暴露VEGF在视网膜各层的表达情况.结果各实验所有视网膜组织中,VEGF的表达随次声暴露的延长而有一定程度的加深.表现为棕黄色阳性染色弥漫分布于神经节细胞层和内核层内.血管分布的区域阳性染色分布较集中.可见表达于内皮细胞的细胞浆内和(或)细胞膜上呈棕黄色圆形,环状分布或不规则分布的颗粒.阴性对照未见阳性染色.结论次声作用导致大鼠视网膜组织VEGF表达不同程度地升高,与次声所致血-视网膜屏障的损害密切相关.

Abstract:

Objective To investigate the possible effect of infrasound on the expression of VEGF in the retina. Methods Twelve mature male rats were used to study the effect of infrasound at 8 Hz frequency and 130 dB sound pressure level. Histological examination