水中运动联合中药内治对糖尿病动脉粥样硬化大鼠动脉细胞凋亡的影响

Effects of aquatic exercise combined with Chinese medicine internal treatment on apoptosis of aortic cells in diabetic atherosclerosis rats

摘要:

目的 探讨水中运动(AE)联合中药内治(CMIT)对糖尿病动脉粥样硬化大鼠动脉细胞凋亡的影响及其可能机制。方法 将125只雄性SD大鼠随机分为空白组、模型组、水中运动组、中药内治组、水中运动联合中药内治组(综合康复组)。除空白组外,各组经链脲佐菌素+高脂饲养构建糖尿病动脉粥样硬化大鼠模型,造模成功后,分别给予模型组、水中运动组、中药内治组、综合康复组生理盐水或中药复方药液2ml灌胃处理,水中运动组与综合康复组在灌胃处理之外,每周进行6次水中运动治疗。各组接受相应处理8周后,检测大鼠血糖、血脂、炎症因子水平,葡萄糖调节蛋白78(GRP78)、CCAAT增强子结合蛋白同源蛋白(CHOP)mRNA表达水平及动脉细胞凋亡指数。采用SPSS 19.0统计软件进行数据分析。多组间比较采用单因素方差分析,2组之间比较采用LSD-t检验。结果 与空白组比较,其余各组血清甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、糖化血红蛋白(GHb)、白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)、C反应蛋白(CRP)、GRP78 mRNA、CHOP mRNA及动脉细胞凋亡指数(CAI)明显升高；高密度脂蛋白胆固醇(HDL-C)明显降低,差异均有统计学意义(均P＜0.01)。与模型组比较,3个治疗组TG、LDL-C、GHb、IL-6、TNF-α、CRP、GRP78 mRNA、CHOP mRNA、CAI明显降低；HDL-C明显升高,差异均有统计学意义(均P＜0.05)。与水中运动组比较,综合康复组TG、LDL-C、GHb、IL-6、TNF-α、CRP、GRP78 mRNA、CHOP mRNA、CAI明显降低；HDL-C明显升高,差异均有统计学意义(均P＜0.05)。 结论 水中运动与中药内治均可调节糖尿病糖脂代谢紊乱及炎症反应,抑制动脉细胞内质网应激,降低动脉细胞凋亡水平,且两者具有协同作用。

Abstract:

Objective To investigate the effect of aquatic exercise combined with Chinese medicine internal treatment on apoptosis of aortic cells in diabetic atherosclerosis rats and the possible mechanism. Methods A total of 125 male SD rats were randomly divided into blank group, model group, aquatic exercise (AE) group, Chinese medicine internal treatment (CMIT) group, aquatic exercise with Chinese medicine internal treatment(AE+CMIT,) group. Except the blank group, the diabetic atherosclerosis model was established in the other groups with the injection of streptozotocin and high-fat feeding. The successfully modeled rats were given 2 ml normal saline or 2 ml Chinese medicine solution respectively. Besides, AE group and AE+CMIT group performed aquatic exercise six times a week. After eight weeks of treatment, blood glucose, blood lipids, inflammatory factors, glucose-regulated protein 78 (GRP78) and CCAAT/enhance-binding protein homologous protein(CHOP) mRNA expression levels and the apoptosis index of arterial cells were measured. SPSS statistics 19.0 was used for data analysis. One-way ANOVA was used for comparison between multiple groups, while LSD-t test was used for comparison between two groups. Results Compared with blank group,triglycerides (TG), low-density lipoprotein cholesterol (LDL-C),glycosylated hemoglobin (GHb), interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), GRP78 mRNA, CHOP mRNA, and cell apoptotic index(CAI) in other groups increased, and high-density lipoprotein cholesterol (HDL-C) decreased, the differences being statistically significant (P＜0.01). Compared with the model group,TG,LDL-C,GHb, IL-6, TNF-α, CRP, GRP78 mRNA, CHOP mRNA, CAI in treatment groups decreased,and HDL-C increased, the differences being statistically significant (P＜0.05). Compared with the AE group, TG, LDL-C, GHb, IL-6, TNF-α, CRP, GRP78 mRNA, CHOP mRNA, and CAI in the AE+CMIT group decreased,and HDL-C increased, the differences being statistically significant (P＜0.05). Conclusion Both aquatic exercise and Chinese medicine internal treatment can regulate metabolism disorder of blood glucose and blood lipid and inflammatory response, inhibit endoplasmic reticulum stress of arterial cells, reduce the level of apoptosis of arterial cells, and they provide a synergistic effect to each other.