乳香通过PTEN/Akt/COX-2通路诱导胃癌细胞SGC7901凋亡

Frankincense induces apoptosis of gastric cancer SGC7901 cells through PTEN/Akt/COX-2 signaling pathway

摘要:

目的 研究乳香对人胃癌细胞SGC7901增殖、凋亡和迁移的影响及其可能的作用机制。方法 不同浓度乳香处理SGC7901细胞不同时间,噻唑兰比色法检测乳香对SGC7901细胞增殖的影响,流式细胞术分析乳香对SGC7901细胞凋亡的影响,蛋白质印迹法(Western blotting)分析乳香对SGC7901细胞中同源性磷酸张力蛋白(PTEN)、磷酸化蛋白激酶B(p-Akt)、蛋白激酶B(PKB,亦称Akt)和环氧化酶2(COX-2)蛋白表达影响,划痕实验检测细胞迁移能力,裸鼠移植瘤实验验证乳香在裸鼠体内抑制肿瘤的效果。采用Graphpad Prism 6.0统计软件对数据进行分析。结果 乳香能够抑制SGC7901细胞的增殖和迁移能力,呈现剂量依赖和时间依赖性。乳香诱导SGC7901细胞的凋亡,呈现剂量依赖性。乳香能够诱导SGC7901细胞中PTEN的表达,抑制p-Akt和COX-2的蛋白表达。此外,乳香能够抑制裸鼠体内胃癌移植瘤的生长。结论 乳香可以抑制胃癌细胞SGC7901的增殖和迁移,诱导细胞凋亡,这可能与PTEN/Akt/COX-2信号通路相关。

Abstract:

ObjectiveTo investigate the effect of frankincense on the proliferation, apoptosis and migration of human gastric cancer SGC7901 cells and the possible mechanisms. Methods After the SGC7901 cells were treated with frankincense at different doses for different time periods, the proliferation of the cells was determined by MTT assay. Flow cytometry was used to detect the apoptosis of the cells treated by 0.0,0.5,1.0,2.0,4.0 or 8.0 mg/ml frankincense for 24 h. Western blotting was employed to detect the expression of phosphatase and tensin homolgy deleted on chromosome ten(PTEN), phosphatased Akt(p-Akt), protein kinase B(PKB, also named Akt), and cyclooxygenase-2(COX-2) in the cells after frankincense treatment (0,2 and 4 mg/ml) for 24 h. Wound-healing test was performed to assess the effect of frankincense on the migration of SGC7901 cells. A xenograft nude mice model of human gastric cancer was established to evaluate the anti-cancer effect of frankincense in vivo. Graphpad Prism 6.0 software was used to perform data analysis. Results Frankincense significantly inhibited the proliferation and migration of SGC7901 cells in a dose-and time-dependent manner. It also induced the apoptosis of the cells dose-dependently. Frankincense treatment resulted in the up-regulation of PTEN and down-regulation of p-Akt and COX-2 at a protein level. It could also inhibit the tumor growth in the xenograft nude mice model of human gastric cancer. Conclusion Frankincense may inhibit the proliferation and migration and promote the apoptosis of SGC7901 cells through the PTEN/Akt/COX-2 signaling pathway.