外周血红细胞表面增强拉曼光谱对老年认知障碍的诊断价值

Diagnositc value of surface-enhanced Raman spectroscopy of peripheral erythrocytes for geriatric cognitive disorder

摘要:

目的 运用表面增强拉曼光谱(SERS)方法对患者外周血红细胞的拉曼光谱进行测量与分析,以探索其对不同认知功能状态老年认知障碍患者的诊断价值。方法 选择上海市第六人民院老年病科住院的患者(年龄>65岁)共62例,均行神经心理量表及临床评估,分为正常对照组、轻度认知障碍(MCI)组、痴呆(AD)组；同时记录基线数据。对红细胞进行SERS测量；运用五阶拟合等方法处理并提取样本拉曼光谱中的有效数据,线性判别法建立数据模型,受试者工作特征曲线检验诊断价值。结果 3组间红细胞SERS存在差异。由红细胞SERS数据得到的对照组与AD组判别模型可正确区分71.8%的病例,灵敏度和特异度分别为91.3%和56.2%；对照组与MCI组判别模型可正确区分58.7%的病例,灵敏度和特异度分别为56.5%和73.9%；AD组与MCI组判别模型可正确区分61.5%的病例,灵敏度和特异度分别为75.0%和65.2%。结论 红细胞SERS数据在认知功能障碍诊断中有一定的价值,可能对研究AD红细胞病理生理变化有所启发。

Abstract:

Objective To investigate the value of surface-enhanced Raman spectroscopy (SERS) of peripheral erythrocytes in the diagnosis of geriatric cognitive disorder. Methods A total of 62 patients (aged >65 years) in the Department of Geriatrics of the Sixth People′s Hospital recieved neuropsychological and clinical assessment. Accordingly, they were divided into normal control (NC) group, mild cognitive impairment (MCI) group, and Alzheimer disease (AD) group. The baseline characteristics were collected and recorded. SERS was performed for erythrocytes. A fifth-order polynomial fitting algorithm was employed to process and extract effective SERS data, linear discriminant method to build a data model, and receiver operating characteristic curve to examine diagnostic value. Results Differences between groups were observed in the SERS of erythrocytes. Discriminant model for NC and AD groups can accuratly distinguish 71.8% patients with a sensitivity of 91.3% and specificity of 56.2%, discriminant model for NC and MCI groups can distinguish 58.7% patients with a sensitivity of 56.5% and specificity of 73.9%, and discriminant model for AD and MCI groups can accuratly distinguish 61.5% patients with a sensitivity of 75.0% and specificity of 65.2%. Conclusion SERS of erythrocytes proves to be of diagnostic value for cognitive impairment, inspiring the research on pathophysiology of erythrocytes in AD.