老年胃癌患者缺氧诱导因子-1α及-2α的表达及其临床意义

Expression of hypoxia inducible factor-1-alpha and -2-alpha in elderly patients with gastric cancer and its clinical significance

摘要:

目的 观察老年胃癌患者缺氧诱导因子-1α(HIF-1α)及HIF-2α的表达情况,并分析其临床意义。方法 选取2017年2月至2018年3月期间在湖北省第三人民医院阳逻院区接受治疗的老年胃癌患者100例,同时选取同期在我院接受胃镜检查的健康老年人(≥65岁)100名作为对照组。免疫组织化学检测2组研究对象HIF-1α及HIF-2α的表达情况。采用SPSS 17.0软件进行数据处理。依据数据类型,组间比较分别采用t检验或χ²检验。多因素logistic回归分析筛选出影响胃癌患者HIF-1α及HIF-2α表达的独立危险因素。结果 胃癌组患者的HIF-1α(85.0% vs 8.0%)及HIF-2α(82.0% vs 5.0%)阳性表达率均显著高于对照组(P<0.001)。多因素logistic回归分析结果显示,淋巴结转移情况(HIF-1α:OR=5.132,95%CI 1.872～14.325,P=0.008；HIF-2α:OR=4.629,95%CI 1.754～16.392,P=0.002)和临床分期(HIF-1α:OR=3.957,95%CI 1.694～8.363,P=0.012；HIF-2α:OR=4.341,95%CI 2.312～10.052,P=0.010)是影响胃癌患者HIF-1α及HIF-2α阳性表达的独立危险因素。结论 老年胃癌患者HIF-1α和HIF-2α阳性表达率较高,且受临床分期和淋巴结转移情况的影响。

Abstract:

Objective To measure the expression of hypoxia inducible factor-1-alpha (HIF-1α) and hypoxia inducible factor-2-alpha (HIF-2α) in the elderly patients with gastric cancer and to analyze its clinical significance. Methods A total of 100 elderly patients with identified gastric cancer treated in the Yangluo Branch from February 2017 to March 2018 were enrolled as the study subjects, and another 100 healthy old individuals (over 65 years old) who received gastroscopy in our branch during the same period were recruited as the control group. The expression of HIF-1α and HIF-2α was detected in the 2 groups. SPSS statistics 17.0 was used to perform the statistical analysis. Student′s t test or Chi-square test was applied for comparison between groups in difference data types. Multivariate logistic regression analysis was employed to screen the independent influencing factors for the expression of the 2 proteins in the patients. Results The positive expression rates of HIF-1α (85.0% vs 8.0%) and HIF-2α (82.0% vs 5.0%) in gastric cancer group were significantly higher than those in control group (P<0.001). Multivariate logistic regression analysis indicated that lymph node metastasis (HIF-1α:OR=5.132,5%CI 1.872-14.325, P=0.008; HIF-2α:OR=4.629, 95%CI 1.754-16.392; P=0.002) and clinical stage (HIF-1α:OR=3.957,5%CI 1.694-8.363, P=0.012; HIF-2α:OR=4.341,5%CI 2.312-10.052, P=0.010) were independent risk factors for the positive expression of HIF-1α and HIF-2α in gastric cancer patients. Conclusion The elderly patients with gastric cancer have higher positive expression rate of HIF-1α and HIF-2α, which may be related with clinical stage and lymph node metastasis.