急性缺血性脑卒中患者外周血红细胞α−突触核蛋白寡聚体浓度的研究

Oligomeric α-synuclein level in peripheral red blood cells of patients with acute ischemic stroke

摘要:

目的 α?突触核蛋白与帕金森病及其他神经退行性疾病相关。动物模型中，细胞缺氧诱发氧化应激反应可导致α?突触核蛋白聚集。急性缺血性脑卒中（AIS）患者外周血红细胞α?突触核蛋白浓度变化未见相关报道。本研究旨在观察AIS患者外周血红细胞α?突触核蛋白寡聚体浓度变化。方法 招募55名正常对照、55名帕金森病患者（PD）和55名AIS患者，并采血留样本。采用纯化的重组α?突触核蛋白双抗体夹心酶联免疫吸附测定法（ELISA）作为进一步检测的测试标准。结果 外周血红细胞α?突触核蛋白寡聚体在正常对照组、PD组及AIS组中分别为（0.41±0.14）、（0.82±0.55）和（1.01±0.56）mmol/L。AIS患者红细胞α?突触核蛋白寡聚体浓度显著高于正常对照组（P＜0.001，95%CI 0.426～0.773）以及PD患者（P＝0.027，95%CI 0.022～0.369）。结论 AIS患者外周血红细胞α?突触核蛋白寡聚体浓度显著升高。这种改变可能参与急性缺血事件的病理生理学改变，但其临床意义有待进一步研究。

Abstract:

Objective Alpha-synuclein has been suggested to be related with the etiology of Parkinson’s disease (PD) and other neurodegenerative disorders. Hypoxia injury has been indicated to be associated with α-synuclein accumulation. But no report has linked the α-synuclein with the pathogenesis of stroke. This study aimed to investigate the level of oligomeric forms of α-synuclein in the patients with acute ischemic stroke (AIS). Methods Fifty-five patients with PD, 55 patients with AIS and 55 healthy individuals were recruited in this study. Their blood samples were characterized by a sandwich ELISA with purified recombinant α-synuclein as a test standard for further detection. Results The level of oligomeric α-synuclein in the red blood cells was (0.41±0.14), (0.82±0.55), and (1.01±0.56)mmol/L respectively for the healthy control, PD and AIS groups. The AIS group had significantly higher α-synuclein level compared with either the healthy controls (P＜0.001, 95%CI＝0.426?0.773) or the PD patients (P＝0.027, 95%CI 0.022?0.369). Conclusion The level of oligomeric α-synuclein in red blood cells is significantly higher in patients with AIS, which may be involved in the pathophysiological changes in AIS, while its clinical significance requires further exploration.