血小板衍生生长因子−C对体外培养血管内皮细胞及间充质干细胞的影响

Platelet-derived growth factor-C promotes proliferation and migration of vessel endothelial cells and mesenchymal stem cells in vitro

摘要:

目的 观察血小板衍生生长因子?C（PDGF-C）对体外培养的血管内皮细胞（VECs）及间充质干细胞（MSCs）的影响。方法 采用培养的大鼠主动脉内皮细胞和MSCs，应用细胞直接计数法、噻唑蓝比色法（MTT法）评价细胞的增殖情况，流式细胞术检测细胞周期，利用Transwell细胞迁移实验和细胞划痕实验测定细胞迁移能力。结果 PDGF-C能明显刺激VECs和MSCs的增殖，增加两种细胞S期细胞的比例，对VECs增殖呈剂量依赖关系，对MSCs的促增殖作用在20μg/L达高峰。同时，PDGF-C促进VECs和MSCs的迁移能力，对MSCs迁移作用强于VECs。结论 PDGF-C可促进培养的VECs、MSCs的增殖与迁移能力。

Abstract:

Objective To determine the effect of platelet-derived growth factor-C (PDGF-C) on vascular endothelial cells (VECs) and mesenchymal stem cells(MSCs) cultured in vitro. Methods The VECs were isolated from thoracic aorta of SD rats and then cultured in vitro. Cryopreserved MSCs were cultured after water bath at 37℃. Cell counting and MTT assay were used to detect the effect of PDGF-C (0, 10, 20, 30, 40 and 50μg/L) on cell proliferation. Flow cytometry was used to detect cell phases. Transwell assay and cell scratch test were used to detect cell migration. Results PDGF-C significantly promoted the proliferation of VECs and MSCs, and increased the percentage of the cells arrested at S phase. Its effect on the VECs proliferation was found in a dose-dependent manner. PDGF-C of 20μg/L promoted the MSCs proliferation at a summit. PDGF-C also promoted the migration ability of cultured VECs and MSCs, especially on MSCs. Conclusion PDGF-C promotes the proliferation and migration of cultured VECs and MSCs.