C反应蛋白单核苷酸多态性与睡眠呼吸暂停综合征患者合并高血压的关系

Correlation of C-reactive protein polymorphism with hypertension in patients with obstructive sleep apnea-hypopnea syndrome

摘要:

目的 探讨C反应蛋白（CRP）单核苷酸多态性与阻塞性睡眠呼吸暂停综合征（OSAHS）合并高血压的关系。方法 2006年1月至2012年12月间于解放军总医院行多导睡眠检查，结果为重度OSAHS的300例男性患者，其中OSAHS伴高血压者145例，并随机抽取100例健康男性对照，采用基因测序的方法，检测CRP启动区基因rs3091244和rs1205的基因多态性。采用R×C列联表χ2检验统计OSAHS伴高血压组、单纯OSAHS组以及健康对照组之间CRP基因多态性的关系。结果 OSAHS合并高血压组rs3091244的CT型基因比例增高，为17%，而单纯OSAHS组为7%。并且rs3091244的T等位基因频率在OSAHS合并高血压组中也明显增高，为9%，而单纯OSAHS组为3%。rs1205的CC基因型在OSAHS合并高血压组中明显升高，为25%，而单纯OSAHS组为11%。结论 CRP的单核苷酸多态性与OSAHS合并高血压有关，rs3091244的CT型基因和rs1205的CC型基因可能通过增加血清CRP水平，引起体内慢性炎症，从而在高血压的发生中产生作用。

Abstract:

Objective To investigate the correlation of single nucleotide polymorphism (SNP) of C-reactive protein(CRP) with hypertension induced by obstructive sleep apnea-hypopnea syndrome (OSAHS). Methods A total of 300 male patients diagnosed as severe OSAHS by polysomnography in our department from January 2006 to December 2012 were enrolled in this study. One hundred and forty-five of them were identified having hypertension. Another 100 healthy males randomly sampled served as normal controls. SNPs of the promoter region of CRP rs3091244 and rs1205 were detected in those OSAHS patients with or without hypertension, and health individuals. Statistical analysis was performed to evaluate the correlation between SNP of CRP and OSAHS with or without hypertension. Results The frequency of CT genotype in CRP rs3091244 was significantly higher in the OSAHS patients with hypertension than those without (17% vs 7%), so was the frequency of T allele in rs3091244 (9% vs 3%). The frequency of CC genotype in CRP rs1205 was also higher in the OSAHS patients with hypertension than those without (25% vs 11%). Conclusion CRP SNP is correlated with hypertension in the OSAHS patients. The CT genotype of CRP rs3091244 and the CC genotype of rs1205 may cause chronic inflammation and promote the occurrence of hypertension through enhancing serum CRP level.