秋水仙碱对烟草侧流烟雾导致的大鼠主动脉氧化应激和内皮功能不全的保护作用

Protective effect of colchicine on aortic oxidative stress and endothelial dysfunction in rats exposed to sidestream cigarette smoke

摘要:

目的 探讨秋水仙碱对大鼠主动脉氧化应激和内皮功能的影响。方法 制备吸入烟草侧流烟雾的大鼠模型，同时通过管饲法给大鼠服用秋水仙碱。56d实验结束后，测定大鼠主动脉中超氧化物歧化酶（SOD）活力，丙二醛（MDA）、一氧化氮（NO）、内皮型一氧化氮合酶（eNOS）含量，以及进行内皮依赖的主动脉舒张实验，观察秋水仙碱对烟草烟雾导致的大鼠主动脉氧化应激和内皮功能改变的影响。结果 与对照组相比，吸入烟草侧流烟雾大鼠组中MDA含量明显升高，NO、eNOS含量以及SOD活力下降，主动脉舒张功能受损，而服用秋水仙碱的大鼠可明显改善这些异常改变（P＜0.05）。结论 （1）吸入烟草侧流烟雾可引起大鼠主动脉内皮氧化应激和内皮功能不全；（2）经秋水仙碱治疗后，可改善大鼠主动脉内皮氧化应激和受损的内皮功能。

Abstract:

Objective To investigate the effect of colchincine on the aortic oxidative stress and endothelial dysfunction induced by exposure to sidestream cigarette smoke (SSCS). Methods The rat model was made by SSCS for 56 consecutive days, and the rats of the intervention group were given colchicine by intragastric injection at the same time. The rats with no smoking exposure and intervention served as normal controls. At the end of treatment, the activity of superoxide dismutase (SOD), and the contents of malondialdehyde (MDA), nitric oxide (NO) and endothelial nitric oxide synthase (eNOS) in the rat aorta were measured. The endothelium-dependent dilation (EDD) test was carried out on the rat aorta. The effect of colchincine on the aortic oxidative stress and endothelial dysfunction was observed. Results Compared with the normal controls, the SSCS model group had significantly increased content of MDA, obviously decreased activity of SOD, and remarkably reduced formation of NO and eNOS, and obviously impaired endothelium-mediated vascular relaxation of aorta. All these phenomena would be attenuated after 56 days’ colchicine treatment. Conclusion Exposure to SSCS induces aortic oxidative stress and endothelial dysfunction in rats. These deleterious effects can be abated by colchicine treatment.