红景天苷对负性心理应激下高强度运动大鼠糖代谢及炎症因子水平的影响

Effect of salidroside on carbohydrate metabolism and serum levels of inflammatory cytokines in rats under negative psychological stress induced by experimental navigation and intensive exercise

摘要:

目的 通过红景天苷对负性心理应激下模拟航渡及高强度运动大鼠的干预，观察干预后大鼠糖代谢及相关炎症因子水平。方法 将6周龄的SD雄性大鼠随机分为静息对照组（A组）、训练对照组（B组）和红景天苷干预组（C组）。每组10只，A组不接受任何刺激，生理盐水灌胃1周后即进行取样，B、C组大鼠经过10d递增负荷跑台训练，分别用生理盐水及红景天苷灌胃1周后，进行模拟航渡及高强度运动，结束即刻取血，测定血糖，并应用酶联免疫吸附分析（ELISA）双抗体夹心法测定相关炎症因子超敏C反应蛋白（hs-CRP），肿瘤坏死因子α（TNF-α），白细胞介素6（IL-6），内皮素1（ET-1）水平。结果 与A组相比, B组血糖和炎症因子hs-CRP，TNF-α，IL-6，ET-1水平均显著升高（P＜0.05）；C组的血糖显著升高（P＜0.05），而hs-CRP, IL-6，ET-1水平均显著降低（P＜0. 05）；与B 组相比, C组hs-CRP，TNF-α，IL-6，ET-1水平均显著降低（P＜0.05），血糖也有降低的趋势。结论 负性心理应激及大强度的运动训练使大鼠糖代谢紊乱，大鼠机体炎症反应增加，炎症因子生成增多，红景天苷能降低应激大鼠机体炎症因子生成，从而改善糖代谢，增强大鼠的抗应激能力。

Abstract:

Objective To determine the effects of salidroside on the carbohydrate metabolism and the serum levels of inflammatory cytokines in rats under negative psychological stress induced by experimental navigation and intensive exercise. Methods A total of 30 6-week Sprague-Dawley rats (SD rats) were randomly and equally allocated into 3 groups, quiescent control group (group A), training control group (group B) and salidroside treatment group (group C). Rats from group A received no stimulation, and administered a 7-day intragastrical injection of normal saline. Rats from group B underwent a 10-day run training with increasing load on treadmill and received 7-day intragastrical administration of normal saline. Group C were subjected to the same procedure of run training as group B, and received 7-day intragastrical administration of 0.4g/kg salidroside. Then, all the rats underwent experimental navigation and intensive exercise. Blood samples were immediately obtained for detection of blood glucose and the levels of inflammatory response-related cytokines, high sensitive-C reactive protein (hs-CRP), tumor necrosis factor α (TNF-α), interleukin 6(IL-6), and endothelin 1 (ET-1) by ELISA. Results As compared with group A, the levels of blood glucose, hs-CRP, TNF-α, IL-6, ET-1 was significantly increased in group B (P<0.05), while, the levels of blood glucose was significantly increased (P<0.05), and those of hs-CRP, IL-6, and ET-1 were significantly decreased in group C (P<0.05). The levels of hs-CRP, TNF-α, IL-6, ET-1 in group C were also significantly decreased than in group B (P<0.05). The level of blood glucose in group C showed a decreasing tendency. Conclusion Negative psychological stress and intensive exercise lead to disorders of glucose metabolism and increase the production of inflammatory cytokines in rats. Salidroside therapy reduces the levels of blood glucose and inflammatory cytokines in rats under stress, and thus improve glucose metabolism and enhances anti-stress capacity.