阿托伐他汀对实验性自身免疫性脑脊髓炎大鼠体内IL-4表达的影响

Effect of atorvastatin on the expression of IL-4 in Wistar rats with experimental autoimmune encephalomyelitis

摘要:

目的 观察不同剂量阿托伐他汀对实验性自身免疫性脑脊髓炎（EAE）神经功能的保护作用，探讨其作用机制。方法 采用Wistar大鼠建立EAE模型，应用不同剂量的阿托伐他汀进行治疗，观察其对大鼠发病情况的影响及程度，并测定白细胞介素（IL）-4在体内的表达及含量变化。结果 大剂量（8mg/kg）治疗组的神经功能最高评分均值和总和评分均值都显著低于EAE模型组[最高评分均值：（1.8±0.2）vs（3.3±0.5），P＜0.05；总和评分均值：（22.2±14.4）vs（42.1±25.7），P＜0.05]，因此，8mg/kg阿托伐他汀治疗可使EAE大鼠神经功能损伤明显减轻，明显改善EAE大鼠的临床症状。大剂量（8mg/kg）阿托伐他汀可明显增加机体内的血清IL-4含量[（0.48±0.35）vs（0.19±0.13）μg/L，P＜0.05]。结论 阿托伐他汀通过增加体内IL-4的含量促进损伤修复，从而起到预防和缓解EAE的作用。

Abstract:

Objective To explore the protective effects of atorvastatin at different doses on the experimental autoimmune encephalomyelitis (EAE) and its underlying mechanism. Methods We induced an EAE model in Wistar rats. After administration, the effect of atorvastatin at different doses on the EAE model was observed, and the serum levels of interleukin 4 were assayed. Results Compared with the EAE model group, the mean of highest score and total score for neurological deficits was significantly lower in atorvastatin treatment group with high dose (8mg/kg) [the mean of highest score: (1.8±0.2) vs (3.3±0.5), P＜0.05; the mean of total score: (22.2±14.4) vs (42.1±25.7), P＜0.05]. High dose Atorvastatin significantly increased the serum level of interleukin 4 [(0.48±0.35) μg/L vs (0.19±0.13) μg/L, P＜0.05]. Conclusion Atorvastatin can prevent and relieve EAE by increasing the serum level of IL-4 to repair injury.