单核细胞趋化蛋白-2518G/A基因多态性与冠心病的关系

MCP-1-2518G/A gene ploymorphisms and coronary atherosclerotic heart disease

摘要:

目的 研究单核细胞趋化蛋白-1（MCP-1）-2518G/A基因多态性对冠心病发病的影响。方法 选取冠心病患者300例, 正常对照组60例。冠心病患者分急性冠状脉综合征（ACS）组180例以及稳定型心绞痛(SAP)组120例。所有患者行冠状动脉造影（CAG）检查, 采用Gensini评分。酶联免疫法测定MCP-1浓度, PCR-RFLP方法检测MCP-1-2518G/A位点多态性, 研究MCP-1-2518G/A基因多态性与冠心病的相关性。结果 （1）MCP-1-2518G/A基因多态性ACS组GG型基因和G等位基因频率分布较SAP组及正常对照组升高[GG型基因: ACS组（33.4%）, SAP组（24.2%）, 对照组（15.0%）, P＜0.05; G等位基因频率: ACS组（51.1%）, SAP组（40.4%）, 对照组（31.7%）, P＜0.05], ACS组AA型基因及A等位基因较SAP组及正常对照组降低[AA型基因: ACS组（31.1%）, SAP组（43.3%）, 对照组（51.7%）, P＜0.05; A等位基因频率: ACS组（48.9%）, SAP组（59.6%）, 对照组（68.3%）, P＜0.05]。（2）三种基因型间MCP-1浓度比较, GG型MCP-1浓度较AG型及AA型升高[GG型基因:（153±22）ng/L, AG型基因:（136±18）ng/L, AA型基因:（124±15）ng/L, （P＜0.05）]。（3）多元回归分析冠脉病变Gensini评分与低密度脂蛋白-胆固醇、空腹血糖、MCP-1 GG型基因成正相关（P＜0.05）, 高密度脂蛋白-胆固醇与冠状动脉狭窄程度呈负相关（P＜0.05）。结论 MCP-1-2518G/A基因多态性与冠心病严重程度相关; MCP-1-2518G/A基因多态G等位基因能通过增强MCP-1表达参与冠心病发病。

Abstract:

Objective To investigate the relationship between MCP-1-2518 G/A gene ploymorphisms and coronary heart disease (CHD). Methods The 360 patients were divided into three groups to undergo the coronary angiography (CAG): acute coronary syndrome (ACS) group (n=180), stable angina pectoris (SAP) group (n=120) and control group (n=60)). The severity and extent of coronary lesions was analyzed by CAG and typified by means of Gensini coronary score system. Enzyme-linked immunosorbent assay was used to measure the MCP-1 concentration. PCR-RFLP way was used to measure MCP-1-2518 G/A gene polymorphisms. Results MCP-1 gene polymorphisms GG gene and G allelic gene was significantly higher in ACS group compared with the SAP group and the control group[GG gene: ACS(33.4%), SAP(24.2%), control group(15.0%), P＜0.05; G allelic gene: ACS(51.1%), SAP(40.4%), control group(31.7%), P＜0.05],and was significantly lower in ACS group compared with the SAP group and the control group[AA gene: ACS(31.1%), SAP(43.3%), control group(51.7%), P＜0.05; A allelic gene: ACS(48.9%), SAP(59.6%), control group(68.3%), P＜0.05]. The MCP-1 concentration in GG gene group was significantly higher than that in AG gene group and AA gene group[GG gene: (153±22)ng/L,AG gene: (136±18)ng/L, AA gene: (124±15)ng/L, (P＜0.05)]. The Gensini score of coronary artery lesions was positively correlated with low density lipoprotein cholesterol, free blood glucose, MCP-1-2518 G/A GG gene polymorphisms (P＜0.05) and high density lipoprotein cholesterol was negatively correlated with stenotic degree of coronary artery.Conclusions MCP-1 gene ploymorphisms is positive regulators of coronary artery lesions. The G allelic of MCP-1-2518 G/A polymorphism can increase the MCP-1 expression than A allelic in cononary heart disease.