人骨髓间充质干细胞的体外多向分化潜能

Multi-directional differentiation of human bone marrow mesenchymal stem cells under diverse induction condition in vitro

摘要:

目的体外观察人骨髓间充质干细胞(hMSCs)向成骨细胞、脂肪细胞及心肌细胞的分化。方法体外培养扩增hMSCs,流式细胞仪分析其免疫表型。取稳定传代的hMSCs,分别在体外向成骨细胞、脂肪细胞及心肌细胞分化,并采用碱性磷酸酶染色、油红O染色及PTAH染色鉴定3种诱导分化后的细胞。结果 hMSCs传代后形态上为典型的成纤维细胞样结构;流式细胞仪检测表明,hMSCs表达CD44、CD105,不表达CD31、CD34和CD45;hMSCs诱导分化后,细胞化学染色显示呈阳性表达的成骨细胞、脂肪细胞和心肌细胞。结论 hMSCs具有多向分化潜能,可向成骨细胞、脂肪细胞和心肌细胞分化。

Abstract:

Objective To observe the differentiation of human bone marrow mesenchymal stem cells (hMSCs) into osteoblasts,adipocytes and cardiomyocytes in vitro.Methods hMSCs were isolated,cultured and passaged in vitro.The phenotypes of hMSCs were identified by flowcytometry.Stably passaged hMSCs were induced to differentiate into osteoblasts,adipocytes and cardiomyocytes in three given induction conditions in vitro.The cytochemistry staining,including alkaline phosphatase staining,oil red O staining and PTAH staining,was utilized to identify the three differentiated cells derived from hMSCs.Results The passaged hMSCs displayed the typical morphological features of fibroblast-like cells.Flow cytometry showed that hMSCs expressed CD44 and CD105,and did not express CD34,CD45 and CD31.Cytochemical staining showed positive staining of osteoblasts,adipocytes and cardiomyocytes differentiated from hMSCs.Conclusion hMSCs have the potential of multi-directional differentiation and can differentiate into osteoblasts,adipocytes and cardiomyocytes in vitro.