罗格列酮和二甲双胍治疗对胰岛素抵抗KKAy糖尿病小鼠肝及横纹肌组织PTEN蛋白表达的影响

Effects of rosiglitazone and metformin on PTEN protein expression in liver and skeletal muscles of insulin resistant diabetic KKAy mice

摘要:

目的观察罗格列酮和二甲双胍治疗后胰岛素抵抗KKAy糖尿病小鼠肝及横纹肌组织PTEN蛋白表达的变化。方法 KKAy小鼠制备糖尿病模型后随机分为未治疗组、罗格列酮治疗组及二甲双胍治疗组。C57BL小鼠普通饲料喂养,为对照组。4周后处死小鼠,Western blot检测肝及横纹肌PTEN蛋白、胰岛素刺激后磷酸化473Akt的表达并进行定量分析。结果罗格列酮及二甲双胍处理后KKAy糖尿病小鼠肝及横纹肌组织磷酸Akt升高倍数与KKAy糖尿病小鼠相比均无明显变化(P>0.05),罗格列酮和二甲双胍处理后肝及骨骼肌PTEN蛋白表达与糖尿病未治疗组相比差异没有统计学意义(P>0.05)。结论罗格列酮和二甲双胍不会影响PI3k-Akt通路活性及PTEN蛋白的表达,两者改善血糖和胰岛素抵抗可能与胰岛素敏感组织PTEN蛋白的表达无关。

Abstract:

Objective To detect the effects of rosiglitazone and metformin on phosphatase and tension homologue deleted on chromosome 10 (PTEN) protein expression in liver and skeletal muscle tissues of insulin resistant diabetic KKAy mice.Methods KKAy mice were fed high fat diet for 4 weeks to prepare diabetic models,which were then randomly divided into 3 groups: diabetic group,rosiglitazone treatment group 12.5 mg/(kg·d),and metformin treatment group 3 g/(kg·d).C57BL mice of 16-week old,fed normal diet,served as control group.After 4 weeks,the mice were sacrificed.Expressions of PTEN protein and phosphoserine Akt in liver and skeletal muscle tissues were determined by Western blot and analyzed quantitatively by gel image analysis instrument.Results Insulin-induced phosphoserine Akt increase in liver and skeletal muscle tissues was not significantly different among rosiglitazone treatment group,metformin treatment group,and KKAy diabetic mice (P>0.05).PTEN protein expression in liver and skeletal muscle tissues was without significant difference among rosiglitazone treatment group,metformin treatment group,and KKAy diabetic mice (P>0.05).Conclusion Rosiglitazone and metformin may not exert any effect on the activity of PI3K-Akt pathway and PTEN protein expression.Their roles in improving insulin resistance and decreasing glucose may be not related with the PTEN expression in insulin sensitive tissue.