人心力衰竭心肌细胞基因表达谱分析方法初探

Preliminary exploration for a method of analyzing gene expression profiles in myocardium cells of heart failure patients

【摘要】目的通过对多中心人类心力衰竭心肌细胞基因表达谱数据整理、分析,探索识别不同表达谱中相同结构蛋白基因的方法。方法选取14个已发表的原发病为扩张型心肌病和(或)缺血性心肌病的心力衰竭基因表达谱,筛选出各表达谱中差异表达≥1.4倍的心肌结构蛋白基因及功能蛋白基因。编写程序自动下载相关基因片段的序列,并建立数据库。根据表达谱中提供的识别号及相关注释信息,用BatchGenAna网站在染色体基因组上进行序列定位,通过定位图显示同一基因不同片段的定位信息。结果通过GenBank序号及芯片中克隆序号得到不同表达谱中相同序号的基因仅有23组,检出率低,漏检了相同基因的不同片段。通过染色体基因组比对、定位,检出不同表达谱中定位在同一染色体区域的相同基因的不同片段共51组,且集中定位在1、2、9、11、12、16号染色体上。通过定位明确显示此基因的编码区及调控区序列。结论基因组定位方法可识别不同表达谱中相同基因的不同片段,明确定位基因编码区及调控区,为后续的功能基因组研究提供可靠信息。

基金项目:国家自然科学基金资助项目(30300130)

【Abstract】Objective To explore a method of identifying the same structural gene in different gene expression profiles of human heart failure myocardium cells. Methods Fourteen available gene expression profiles in myocardium of heart failure patients were selected. These patients suffered dilated cardiomyopathy and/or ischemic cardiomyopathy. Over 1.4 times differentially expressed structural and functional genes were screened out. A local database of these genes with known nucleotide sequences was established. According to the Accession Number and other information of genes provided in the profiles,the genomic location of these genes on the chromosome was performed by BatchGenAna. The location information of different fragments of the same gene was shown in location map. Results Only 23 groups of genes were obtained by the Accession Number in distinct profiles,and the different fragments of the same gene were left out. By sequence comparison and genomic location,51 groups of genes were identified,including different fragments of the same gene. The genes mostly distributed on chromosome 1,2,9,11,12,16. The coding region and regulation region were also distinguished. Conclusion Genomic location is effective in identifying the different fragments of the same gene,and in locating definitely the coding region and transcriptional regulation region. The accurate sequences of genes in these regions will provide reliable information for further investigation of the functional genomics.?更多