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解放军总医院医学创新研究部、国家老年疾病临床医学研究中心(解放军总医院)、解放军总医院第六医学中心心血管病医学部
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中华老年多器官疾病杂志编辑委员会
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E-mail: zhlndqg@mode301.cn
创刊人 王士雯
主 编 范利
执行主编 陈韵岱
编辑部主任 王雪萍
ISSN 1671-5403
CN 11-4786
创刊时间 2002年
出版周期 月刊
邮发代号 82-408
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张丽芬,田曜恺.化痰除湿汤对衰老大鼠胸腺皮质超微结构和氧化损伤的影响[J].中华老年多器官疾病杂志,2022,21(1):49~54
化痰除湿汤对衰老大鼠胸腺皮质超微结构和氧化损伤的影响
Effect of Huatan Chushi Decoction on thymus cortex ultrastructure and oxidative damage in aging rats
投稿时间:2021-04-20  
DOI:10.11915/j.issn.1671-5403.2022.01.011
中文关键词:  衰老  抗氧化力  胸腺淋巴细胞  胸腺上皮细胞  化痰除湿汤
英文关键词:aging  total antioxidation capacity  thymus epithelial cells  thymus lymphocytes  Huatan Chushi Decoction Corresponding author:ZHANG Li-Fen, E-mail:zhang.lifen@bucm.edu.cn〖FL
基金项目:
作者单位E-mail
张丽芬 北京中医药大学第三附属医院肾病科,北京 100029 zlf13601056119@163.comeffect 
田曜恺 广州中医药大学第二临床医学院,广州510006 zlf13601056119@163.comeffect 
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中文摘要:
      目的 研究化痰除湿汤对衰老大鼠胸腺皮质超微结构和氧化损伤的作用。方法 选择体质量130~170g Wistar大鼠40只,随机分为4组(n=10):正常对照组为颈背皮下注射1ml/(kg·d)生理盐水,灌胃10ml/(kg·d)蒸馏水;衰老组为颈背皮下注射D-半乳糖溶液48mg/(kg·d),灌胃10ml/(kg·d)蒸馏水;化痰除湿组为颈背皮下注射D-半乳糖溶液48mg/(kg·d),灌胃化痰除湿汤水煎剂20g/(kg·d);阳性对照组为颈背皮下注射D-半乳糖溶液48mg/(kg·d),灌胃维生素E溶液0.3g/(kg·d)。共干预40d。完成干预后,颈椎脱臼法处死大鼠,检测血清总抗氧化力(T-AOC)、乳酸脱氢酶(LDH)及脂质过氧化物(LPO)含量;透射电镜观察胸腺皮质上皮细胞和淋巴细胞超微结构。采用SPSS 20.0统计软件进行数据分析。多组间比较采用单因素方差分析(One-way ANOVA),组间两两比较采用Bonferroni检验。P<0.05为差异有统计学意义。结果 实验14、28、41d各组大鼠体质量均有明显增长,化痰除湿组体质量增长率较其他3组略低,但各组间比较,差异均无统计学意义(均P>0.05)。治疗第41天,与正常对照组比较,衰老组大鼠血清T-AOC水平显著降低,血清LDH、LPO水平显著升高,差异均有统计学意义(均P<0.01)。与衰老组比较,化痰除湿组和阳性对照组血清T-AOC水平均升高,血清LDH水平显著下降,差异均有统计学意义(均P<0.05);化痰除湿组血清LPO水平显著下降,差异有统计学意义(P<0.01);阳性对照组血清LPO水平也有降低,但差异无统计学意义(P>0.05)。与阳性对照组比较,化痰除湿组血清T-AOC水平显著较高,血清LPO水平显著较低,差异均有统计学意义(均P<0.01);2治疗组血清LDH比较,差异无统计学意义(P>0.05)。衰老组胸腺皮质中上皮细胞和淋巴细胞明显减少,细胞结构破坏明显,细胞核皱缩变形,胞质内线粒体和内质网等细胞器明显减少,溶酶体增多;化痰除湿组和阳性对照组胸腺皮质中上皮细胞和淋巴细胞较衰老组增多,细胞结构较衰老组明显改善。结论 化痰除湿汤可显著提高机体抗氧化力,减轻氧化损伤及减少氧化产物,增加胸腺皮质上皮细胞和淋巴细胞数量,保护细胞形态和线粒体超微结构。化痰除湿汤有干预性抗衰老和提高免疫作用。
英文摘要:
      Objective To study the effect of Huatan Chushi Decoction on the ultrastructure of thymus cortex and oxidative damage in aging rats. Methods A total of 40 Wistar rats (weighing 130-170 g) were randomly divided into normal control group, aging group, decoction group and positive control group, with 10 rats in each group. After receiving subcutaneous injection at nape of neck with 1 ml/(kg·d) normal saline in the normal control group and 48 mg/(kg·d) D-galactose in the other 3 groups, the rats of above groups were gavaged distilled water at 10 ml/(kg·d) in the former 2 groups, 20 g/(kg·d) decoction in the decoction group, and 0.3 g/(kg·d) vitamin E solution in the positive control group. At the end of 40 days′ intervention, all rats were sacrificed, and the contents of total antioxidation capacity (T-AOC), lipid peroxide(LPO) and lactate dehydrogenase (LDH) in the serum were detected. The ultrastructure of thymus cortex was observed with transmission electron microscopy. SPSS statistics 20.0 was used to perform the statistical analysis. One-way analysis of variance was applied for multi-group comparison, and Bonferroni test for comparison between groups. P<0.05 was regarded as statistical significance. Results The body mass of rats in each group was increased significantly on the 14th, 28th and 41st days, and the growth rate was slightly lower in the decoction group than the other 3 groups, but there was no significant difference among the groups (all P>0.05). On the 41st day, the T-AOC level was significantly lower while the contents of LDH and LPO were obviously higher in the aging group than the control group (P<0.01). The T-AOC level was remarkably increased while the content of LDH was notably decreased in the decoction group and positive control group than the aging group (P<0.05). The serum LPO content was decreased in the decoction group and the positive control group, but statistical difference was only seen in the former group (P<0.01). The decoction group had higher T-AOC level but lower LPO content when compared with the positive control group (P<0.01), but no such difference was observed in the content of LDH (P>0.05). In the aging group, the epithelial cells and lymphocytes in the thymus cortex were significantly reduced, and displayed obviously destroyed cell structure, with shrunk and deformed nucleus, decreased mitochondria, endoplasmic reticulum and other organelles and increased lysosomes in the cytoplasm. The decoction group and the positive control group showed larger numbers of epithelial cells and lymphocytes in the thymus cortex and obviously improved cell structure when compared with the aging group. Conclusion The decoction can improve the body′s antioxidant capacity, reduce oxidative damage and accumulation of oxidative products, increase the numbers of epithelial cells and lymphocytes in the thymus cortex, and protect cell morphology and mitochondrial ultrastructure. Huatan Chushi Decoction has an intervening anti-aging and immune function
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